anti iba1  (Cell Signaling Technology Inc)

Journal: bioRxiv

Article Title: 5xFAD-NSG mice: a preclinical Alzheimer model for human cell therapy studies

doi: 10.64898/2026.03.03.709048

Figure Lengend Snippet: Microglia activity increases with age in 5xFAD-NSG mouse brains. A. Representative immunohistochemical (IHC) staining of Iba1 antibody showing increased microglia in different brain regions from 21-week old 5xFAD-NSG mouse relative to microglia observed in non-Tg littermate (NTL). Scales each represent 300 μm. B. Higher magnification IHC images of Iba1 antibody showing microglia morphology changes in 5xFAD-NSG mice at 13, 21, and 35 weeks of age. Scales each represent 50 μm. C. Quantification of the extent of Iba1-labeled microglia by age in the forebrain shows microglia are increased in 5xFAD-NSG mice at 13-(n = 4), 21-(4), and 26-week (4) relative to NTL (6). D. Quantification of the extent of Iba1-labeled microglia by age in the cerebral cortex shows microglia are increased in 5xFAD-NSG mice at 13-(n = 3), 21-(4), and 26-week (4) relative to NTL (6). Median shown by horizonal line, p value determined by Dunn’s multiple comparison test 5xFAD-NSG ages relative to NTL. The NTL group was combined from 2 mice from each age group. ns means not significant.

Article Snippet: Heat induced epitope retrieval using Bond Epitope Retrieval 2, pH9 (Leica Biosystems AR9640) was incubated at 100°C for 20-30 min. Primary antibodies were applied at room temperature (RT) with the following parameters: Beta-Amyloid antibody (Thermo 700254) incubated for 15 min at RT at a 1:100 dilution; and Iba1 antibody (Cell Signaling 17198) incubated for 15 min at a 1:500 dilution.

Techniques: Activity Assay, Immunohistochemical staining, Immunohistochemistry, Labeling, Comparison